Primer design

Description

Primer design is the strategic selection and synthesis of short, single-stranded DNA or RNA sequences, known as primers, which are essential for initiating the polymerase chain reaction (PCR) or DNA sequencing reactions.

Effective primer design involves meticulous consideration of factors such as target specificity, melting temperature, and secondary structure to ensure accurate and efficient amplification or sequencing of the desired DNA region.

This tool is built on top of the Primer3 and primer3-py Python wrapper.

Methods

PickPCRPrimers

Designs primers for a PCR reaction using Primer3.

Models

Primer

Primer and it's key paramets.

PrimerPair

A pair of primers for running a PCR.

PCRConcentrations

Concentrations of the key components of the PCR reaction.

Parameter3Range

Parameter range defined by a minimal, maximal and optimal values.

Parameter2Range

Parameter range defined by minimal and maximal values.

Interval

Sequence interval defined by a start position and lenght in bp.